EVALUATION OF INDOPHENOL DERIVATIVES AS REAGENTS FOR DEMONSTRATING CYTOCHROME OXIDASE IN TISSUE SECTIONS

Direct immunoperoxidase method for demonstrating Chlamydia psittaci in tissue sections.

Partial inactivation of cytochrome c oxidase by nonpolar mercurial reagents.

Purified beef heart cytochrome c oxidase is inactivated to the extent of 35 to 50% by the nonpolar mercurial reagents mercuric chloride and ethylmercuric chloride. The inactivation is complete within 5 min. In titrations of activity, the plateau level of inactivation is attained at added ethylmercuric chloride:heme a ratios of about 1:1. Up to 3 mercury atoms/heme a are bound to the oxidase, al...

Reversible Inactivation of Cytochrome Oxidase by Disulfide Bond Reagents.

Recent studies on the chemical nature of cytochrome oxidase (cytochrome c :02 oxidoreductase, EC 1.9.3.1) have been concerned largely with the structure of its iron-porphyrin prosthetic group (cj. (l)), the importance of copper as an integral component of the enzyme (e.g. (2-5)), and the possible role of a lipid factor (e.g. (6-8)). By contrast, little work has been done on the nature of the fu...

Influence of sulfhydryl reagents on the cytochrome c-cytochrome oxidase system.

Earlier papers (1,2) from this laboratory demonstrated that basic phenylmercuric nitrate depressed the cytochrome c-cytochrome oxidase system, determined manometrically, as well as yeast respiration (3) and bacterial growth (4), and that protection against these depressions could be afforded by sulfhydryl compounds (24) which, however, would not reverse the depression once it was established. I...

Tissue cytochrome oxidase activity and body weight.

Recently there has been evidence of an increasing amount of interest in the enzyme systems or factors responsible for the level of the basal heat production in the intact animal (1, 2). Krebs (2) and von Bertalanffy and Pirozynski (3) have extended the studies of Kleiber and coworkers (4, 5) in which the Qo, of surviving tissue slices was measured and related to body size. In interspecific stud...